Paraffin-embedding for large volume bio-tissue
نویسندگان
چکیده
منابع مشابه
A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins.
Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice. Tissues from these mice are commonly formalin fixed and paraffin embedded (FFPE) for histological studies. However, this results in inactivation of the natural fluorescence of these proteins, requiring their detection by immunolo...
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Benchmarks Fixed tissues often need to be arranged in a particular configuration , order, or orientation prior to processing for paraffin embedding. Although it is possible to arrange tissues after infiltration (during the final stages of paraffin embedding), it can be more convenient and practical to do this at the time of dissection or tissue collection. Agar has been the classical medium use...
متن کاملParaffin embedding technique for specimens obtained by vitrectomy.
Histologic and immunohistochemical preparation of pathologic fluids containing cellular material is difficult, because the techniques are limited by the amount of cellular material. Similar problems limit the preparation of vitrectomized material. Cytopreparatory techniques, including millipore filter and celloidin bag cell block, have been introduced since 1980 for diagnostic vitrectomies. The...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-68876-5