Paraffin-embedding for large volume bio-tissue

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins.

Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice. Tissues from these mice are commonly formalin fixed and paraffin embedded (FFPE) for histological studies. However, this results in inactivation of the natural fluorescence of these proteins, requiring their detection by immunolo...

Using word embedding for bio-event extraction

Bio-event extraction is an important phase towards the goal of extracting biological networks from the scientific literature. Recent advances in word embedding make computation of word distribution more efficient and possible. In this study, we investigate methods bringing distributional characteristics of words in the text into event extraction by using the latest word embedding methods. By us...

A Rapid Method of Paraffin Embedding

Agar-gelatin for embedding tissues prior to paraffin processing.

Benchmarks Fixed tissues often need to be arranged in a particular configuration , order, or orientation prior to processing for paraffin embedding. Although it is possible to arrange tissues after infiltration (during the final stages of paraffin embedding), it can be more convenient and practical to do this at the time of dissection or tissue collection. Agar has been the classical medium use...

Paraffin embedding technique for specimens obtained by vitrectomy.

Histologic and immunohistochemical preparation of pathologic fluids containing cellular material is difficult, because the techniques are limited by the amount of cellular material. Similar problems limit the preparation of vitrectomized material. Cytopreparatory techniques, including millipore filter and celloidin bag cell block, have been introduced since 1980 for diagnostic vitrectomies. The...